| Auburndale Youth Soccer Club | | | | | | | | | | | | |
| Bartow SC | | | | | | | | | | | | |
| Braden River | | | | | | | | | | | | |
| Celtic FC | | | | | | | | | | | | |
| Central Academy Youth Soccer Association | | | | | | | | | | | | |
| Chargers Soccer Club | x | | | | | | | | | | | |
| Charlotte County Soccer Federation | x | | | | | | | | | | | |
| East Pasco YSL | x | | | | | | | | | | | |
| Englewood Youth Soccer Association | | | | | | | | | | | | |
| FC Sarasota | | | | | | | | | | | | |
| FC Tampa Rangers | x | | | | | | | | | | | |
| Fishhawk YSC | | | | | | | | | | | | |
| Florida Premier | x | | | | | | | | | | | |
| Florida Tropics | | | | | | | | | | | | |
| Lake Wales SC | | | | | | | | | | | | |
| Lee County Strikers | | | | | | | | | | | | |
| Manasota Youth Soccer League | | | | | | | | | | | | |
| Manatee Area YSA | | | | | | | | | | | | |
| Nature Coast Soccer Club | x | | | | | | | | | | | |
| Northport Fusion FC | | | | | | | | | | | | |
| Pinellas County United FC | x | | | | | | | | | | | |
| Plant City FC | | | | | | | | | | | | |
| Players Club of Tampa Bay | | | | | | | | | | | | |
| Rural YSA | | | | | | | | | | | | |
| San Carlos Park Scorpion SC | | | | | | | | | | | | |
| Sarasota Spartans | x | | | | | | | | | | | |
| Seminole Shooting Stars SA | | | | | | | | | | | | |
| St Pete Raiders | | | | | | | | | | | | |
| Tampa Dynamo | | | | | | | | | | | | |
| Venice Falcons SC | | | | | | | | | | | | |
| West Citrus | | | | | | | | | | | | |
| Winter Haven Youth Soccer | x | | | | | | | | | | | |
